fixation solution true-nuclear transcription factor buffer set (Thermo Fisher)

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Thermo Fisher fixation solution true-nuclear transcription factor buffer set
Fixation Solution True Nuclear Transcription Factor Buffer Set, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixation+buffer+solution/fixation+solution+true+nuclear+transcription+factor+buffer+set/pmc11489549__mmc2-390-7-17
Average 90 stars, based on 1 article reviews

fixation solution true-nuclear transcription factor buffer set - by Bioz Stars, 2026-09

90/100 stars

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Suspension:

Article Title: Divergent somatic mutation patterns among human cerebellar neuron types
Article Snippet: Nuclei were imaged on a hemocytometer with a light microscope after staining with a 0.2% final concentration of trypan blue (Thermo Scientific, 15250061), and for samples with many nuclei clumps, we performed additional pipette mixing 30 to 50 times with wide-bore 1000 μL tips and passed the nuclei again through a 30 μm MACS SmartStrainer filter. .. We measured the volume of the nuclei suspension, we added an equal volume of 2% PFA fixation solution (0.25 M sucrose, 5 mM calcium chloride, 3 mM magnesium acetate, 0.1 mM EDTA, 10 mM Tris-HCl pH 7.5, 2% PFA [from Thermo Fisher, 28906]) to achieve a final concentration of 1% PFA, and we placed the sample on a rotator for 10 min at room temperature. .. We then added glycine quenching solution (2.5 M glycine [Sigma-Aldrich, 50046] in NFW) to the fixed nuclei to a final concentration of 0.2 M glycine, and incubated on a rotator at room temperature for 10 min.

Concentration Assay:

other:

Article Title: ANV600 is a novel PD-1 targeted IL-2Rβγ agonist that selectively expands tumor antigen-specific T cells and potentiates PD-1 checkpoint inhibitor therapy.
Article Snippet: Cells were fixed and permeabilized using the FoxP3 Fixation/Permeabilization working solution from the FoxP3 Transcription Factor Staining Buffer Set (eBioscience).

Article Title: Unraveling the role of shrimp hydrolysate as a food supplement in the immune function and fecal microbiota of beagle dogs.
Article Snippet: The PBMC were washed with FACS buffer, fixed with Foxp3 Fixation/Permeabilization solution (eBioscience) for 45 min, and permeabilized using Foxp3 Permeabilization Buffer (eBioscience).

Incubation:

Article Title: The Oxytocin Receptor in Spermatozoa May Originate From Both Spermatogenesis and Epididymal Maturation, and Regulates Capacitation.
Article Snippet: Two hundredmicroliter of each sperm sample was incubated with 1 μL LIVE/DEAD (LD) violet working solution (Thermo Fisher Scientific, Massachusetts, United States) at 38◦C for 30 min in the dark, and then centrifuged at 650 × g for 5 min. .. Samples were subsequently resuspended in fixation solution (containing 4% paraformaldehyde, Thermofisher, Kandel, Germany; 0.1% Triton X100 and 0.3% Tween-20) and incubated in a rotating shaker at room temperature for 30 min. ..

Article Title: The Oxytocin Receptor in Spermatozoa May Originate From Both Spermatogenesis and Epididymal Maturation, and Regulates Capacitation
Article Snippet: Two hundred microliter of each sperm sample was incubated with 1 μL LIVE/DEAD (LD) violet working solution (Thermo Fisher Scientific, Massachusetts, United States) at 38°C for 30 min in the dark, and then centrifuged at 650 × g for 5 min. .. Samples were subsequently resuspended in fixation solution (containing 4% paraformaldehyde, Thermofisher, Kandel, Germany; 0.1% Triton X‐100 and 0.3% Tween‐20) and incubated in a rotating shaker at room temperature for 30 min. ..

Article Title: The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stress.
Article Snippet: Two hundred μL of each sperm sample was incubated with 1 μL LIVE/DEAD (LD) violet working solution (Thermo Fisher Scientific, Waltham, MA, USA) at 38 ◦C for 30 min in the dark, and then centrifuged at 660 g for 5 min. .. Samples were subsequently resuspended in a fixation solution (4 % paraformaldehyde; Thermofisher, Kandel, Germany) containing 0.1 % Triton X-100 and 0.3 % Tween-20, and incubated in a rotating shaker at room temperature for 30 min. ..

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